Aging Cell-size Dependent Lipofuscin Accumulation Anaysis Using a Novel Microfluidic Microfilter with Uniform Fluidic Profile

Throughput enhancement is an important issue for processing large volume of sample, especially for microfilters. As a simple and straightforward method, expansion of channel width can handle larger volume without flow velocity increase. The non-uniform velocity distribution by parabolic profile, however, causes different fluidic force to captured cells in microfilters depending on the position of the wide microchannel. We have developed a novel diamond structure-based microfluidic microfilter to form uniform flow velocity profile in the whole area of microfilters and analyzed the lipofuscin accumulation of aged fibroblasts depending on their cell sizes.